Commonly this pressure is 0.25 inches of water column, but could be lifted or decreased as the procedure demands. Isolators have to be periodically leak tested to be sure their integrity and stop escape on the decontamination agent. Leak testing can be carried out by several solutions including tension https://kameronigcwr.fliplife-wiki.com/295066/little_known_facts_about_containment_aseptic_isolator